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KMID : 0380219900230040510
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Journal of Biochemistry and Molecular Biology
1990 Volume.23 No. 4 p.510 ~ p.513
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A Simple Spectrophotometric Assay Method for Hydrogenase
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Choi Suk-Jung
Yang Chul-Hak
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Abstract
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A spectrophotometric assay method for hydrogenise was studied. The sample solution was flushed with nitrogen or hydrogen to remove oxygen, and the reaction started. At the end of the reaction, 2´,3´,5´-tilhhenyl tetrazolium chloride was reduced with reduced methyl viologen, and the concentration was determined by monitoring the absorbance at 600 nm. "I´he absorbance increased linearly with increasing amount of enzyme in both cases of uptake and evolution assay. Alternatively, the concentration of reduced MV can be measured directly with autofill or microflow device which avoids a contact of the solution with air. This method also showed good results.
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KEYWORD
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